Yuxuan Guo, PI

Contact Information

Guo@@bjmu.edu.cn

11th Floor, North Tower, Peking University Medical Science Building

Research Group Website: https://www.x-mol.com/groups/yuxuan_guo

Research Interests

1. Development of gene therapy tools for cardiovascular diseases based on adeno-associated virus (AAV) vectors.

2. Investigation of molecular mechanisms underlying cardiac development, repair, and regeneration.

3. Development and evaluation of novel cardiac biotherapeutic strategies using animal and human-derived models.

Education and Professional Experience

2006.9-2010.7 School of Life Sciences, Peking University, Bachelor's Degree

2010.8-2015.8 Department of Biology, Johns Hopkins University, Ph.D.

2015.9-2020.11 Boston Children's Hospital/Harvard Medical School, Postdoctoral Fellow

2020.11-Present Principal Investigator (PI), Peking University School of Basic Medical Sciences/Institute of Cardiovascular Sciences

Publications (*Corresponding author/#First author)

1. Research Articles (10 representative papers)

Chen G, Zhang Y, Liu Z, Wu J, Chen Z, Yang L, Zhang J, Wu Y, Li J, Bai B, Lv Z, Gao F*, Dong E*, Guo Y*. Myocardial infarction creates a critical time window for AAV-based cardiac gene transfer.  Fundamental Research , 2025.

Chen Z#, Yang L#, Zhang Y, Li J, Yang Y, Li Y, Fan L, Chen W, Miao L, Liu J, Chen G, Wang Z, Li Y, Gao F, Zhou J, Zheng L, Zhang Y, Zhao D, Pu WT, Yang K, Dong E*, Guo Y*. The drug-elicitable alternative splicing module for tunable vector expression in the heart.  Nat Cardiovasc Res . 2025 Jun 13. doi: 10.1038/s44161-025-00665-7. (with editorial)

Liu Z#, Yang L#, Yang Y, Li J, Chen Z, Guo C, Guo Q, Li Q, Zhao D, Hu X*, Gao F*, Guo Y*. ABE-Mediated Cardiac Gene Silencing via Single AAVs Requires DNA Accessibility.  Circ Res . 2025 Jan 31;136(3):318-320. doi: 10.1161/CIRCRESAHA.124.325611.

Sun Y, Guo C, Chen Z, Lin J, Yang L, Zhang Y, Wu C, Zhao D, Jardin B, Pu WT, Zhao M, Dong E, Hu X, Zhang S*, Guo Y*. Non-Cell-Autonomous Cardiomyocyte Regulation Complicates Gene Supplementation Therapy for Lmna-Associated Cardiac Defects in Mice.  JACC Basic Transl Sci . 2024 Aug 21;9(11):1308-1325. doi: 10.1016/j.jacbts.2024.06.004. (with editorial)

Yang L#, Liu Z#, Chen G, Chen Z, Guo C, Ji X, Cui Q, Sun Y, Hu X, Zheng Y, Li Y, Gao F, Chen L, Zhou P, Pu WT, Guo Y*. MicroRNA-122-Mediated Liver Detargeting Enhances the Tissue Specificity of Cardiac Genome Editing.  Circulation . 2024 May 28;149(22):1778-1781. doi: 10.1161/CIRCULATIONAHA.123.065438.

Guo Y*#, Cao Y#, Jardin BD#, Zhang X, Zhou P, Guatimosim S, Lin J, Chen Z, Zhang Y, Mazumdar N, Lu F, Ma Q, Lu YW, Zhao M, Wang DZ, Dong E, Pu WT*. Ryanodine receptor 2 (RYR2) dysfunction activates the unfolded protein response and perturbs cardiomyocyte maturation.  Cardiovasc Res . 2023 Mar 17;119(1):221-235. doi: 10.1093/cvr/cvac077.

Guo Y*#, Cao Y#, Jardin BD#, Sethi I, Ma Q, Moghadaszadeh B, Troiano EC, Mazumdar N, Trembley MA, Small EM, Yuan GC, Beggs AH, Pu WT*. Sarcomeres regulate murine cardiomyocyte maturation through MRTF-SRF signaling.  Proc Natl Acad Sci U S A . 2021 Jan 12;118(2):e2008861118. doi: 10.1073/pnas.2008861118.

Guo Y, Jardin BD, Zhou P, Sethi I, Akerberg BN, Toepfer CN, Ai Y, Li Y, Ma Q, Guatimosim S, Hu Y, Varuzhanyan G, VanDusen NJ, Zhang D, Chan DC, Yuan GC, Seidman CE, Seidman JG, Pu WT. Hierarchical and stage-specific regulation of murine cardiomyocyte maturation by serum response factor.  Nat Commun . 2018 Sep 21;9(1):3837. doi: 10.1038/s41467-018-06347-2.

Guo Y#, VanDusen NJ#, Zhang L, Gu W, Sethi I, Guatimosim S, Ma Q, Jardin BD, Ai Y, Zhang D, Chen B, Guo A, Yuan GC, Song LS, Pu WT. Analysis of Cardiac Myocyte Maturation Using CASAAV, a Platform for Rapid Dissection of Cardiac Myocyte Gene Function In Vivo.  Circ Res . 2017 Jun 9;120(12):1874-1888. doi: 10.1161/CIRCRESAHA.116.310283. Epub 2017 Mar 29. (with editorial)

Guo Y, Kim Y, Shimi T, Goldman RD, Zheng Y. Concentration-dependent lamin assembly and its roles in the localization of other nuclear proteins.  Mol Biol Cell . 2014 Apr;25(8):1287-97. doi: 10.1091/mbc.E13-11-0644. Epub 2014 Feb 12. (Highlight from MBoC Selection)

2. Other Articles

Wang Z#, Wu J#, Lv Z#, Liang P*, Li Q*, Li Y*, Guo Y*. LMNA-related cardiomyopathy: From molecular pathology to cardiac gene therapy.  J Adv Res . 2025 Jan 17:S2090-1232(25)00001-3. doi: 10.1016/j.jare.2025.01.001.

Guo C, Ma X, Gao F*, Guo Y*. Off-target effects in CRISPR/Cas9 gene editing.  Front Bioeng Biotechnol.  2023 Mar 9;11:1143157. doi: 10.3389/fbioe.2023.1143157. (ESI Highly Cited Paper)

Guo Y#, Pu WT*. Cardiomyocyte Maturation: New Phase in Development.  Circ Res . 2020 Apr 10;126(8):1086-1106. doi: 10.1161/CIRCRESAHA.119.315862. (ESI Highly Cited Paper)

Guo Y, Pu WT. Genetic Mosaics for Greater Precision in Cardiovascular Research.  Circ Res . 2018 Jun 22;123(1):27-29. doi: 10.1161/CIRCRESAHA.118.313386.

Galdos FX#, Guo Y#, Paige SL#, VanDusen NJ#, Wu SM, Pu WT. Cardiac Regeneration: Lessons From Development.  Circ Res . 2017 Mar 17;120(6):941-959. doi: 10.1161/CIRCRESAHA.116.309040.

Teaching

1. Undergraduate Courses

Frontiers in Basic Medicine I ------ Gene Editing

Research Methods in Cardiovascular Diseases ------ Gene Therapy

Scientific Research Thinking Training ------ Cardiovascular Research

Physiology Laboratory

2. Graduate Courses

Advances in Molecular Biology ------ Gene Editing

Cardiac Pathophysiology ------ Cardiac Development, Disease, and Regeneration

Literature Reading and Writing I ------ Rapid Literature Reading Training

Literature Reading and Writing II ------ Scientific Presentation (PPT) Preparation

Cytoskeleton and Biomechanics ------ Intermediate Filaments and Nuclear Mechanics

Molecular Pharmacology ------ Gene Therapy

Design and Biological Application of Nanotheranostic Systems ------ Viral Vectors

Research Funding

1. National Natural Science Foundation of China (NSFC)

2022-2024 Young Scientist Fund Project C, PI

2022-2025 General Program, PI

2023-2025 Young Scientist Fund Project B, PI

2026-2029 General Program, PI

2. National Key R&D Program of China and National Major Science and Technology Projects

2022-2027 Stem Cell and Organ Repair Special Project, Project Leader

2024-2028 "Four Major Chronic Diseases" Special Project, Key Participant

3. Beijing Municipal Science and Technology Plan and Beijing Natural Science Foundation

2021-2024 Beijing Nova Program, PI

2022-2024 Nova Program Cross-Disciplinary Project, Lead PI

2023-2025 General Program, PI

2025-2028 Cross-Integration Non-Consensus Innovation Project, PI